dynamic array integrated fluidic circuit ifc chips (fluidigm)
94
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fluidigm
dynamic array integrated fluidic circuit ifc chips
Dynamic Array Integrated Fluidic Circuit Ifc Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48%2E48+dynamic+array+chips/48%2E48+Dynamic+Array+IFC+for+Gene+Expression/pm41866099-141-7-22
Average 94 stars, based on 313 article reviews
Dynamic Array Integrated Fluidic Circuit Ifc Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/48%2E48+dynamic+array+chips/48%2E48+Dynamic+Array+IFC+for+Gene+Expression/pm41866099-141-7-22
Average 94 stars, based on 313 article reviews
dynamic array integrated fluidic circuit ifc chips - by Bioz Stars,
2026-10
94/100 stars
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Real-time Polymerase Chain Reaction:Article Title: P2x7 receptors control demyelination and inflammation in the cuprizone model Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Article Title: Apparatus for magnetic separation of cells Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Article Title: REST represses a subset of the pancreatic endocrine differentiation program Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Reverse Transcription:Article Title: P2x7 receptors control demyelination and inflammation in the cuprizone model Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Single Cell:Article Title: Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis Article Snippet: Reverse transcription and low cycle pre-amplification was performed following addition of Superscript III reverse transcriptase enzyme (Invitrogen, Waltham, MA, USA), Cells Direct reaction mix (Invitrogen), and target gene-specific TaqMan assay (primer/probe) sets (Applied Biosystems) ( ). .. Resultant single cell cDNA was mixed with sample loading agent (Fluidigm, South San Francisco, CA, USA) and Universal PCR Master Mix (Applied Biosystems) and loaded into 48.48 Dynamic Polymerase Chain Reaction:Article Title: Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis Article Snippet: Reverse transcription and low cycle pre-amplification was performed following addition of Superscript III reverse transcriptase enzyme (Invitrogen, Waltham, MA, USA), Cells Direct reaction mix (Invitrogen), and target gene-specific TaqMan assay (primer/probe) sets (Applied Biosystems) ( ). .. Resultant single cell cDNA was mixed with sample loading agent (Fluidigm, South San Francisco, CA, USA) and Universal PCR Master Mix (Applied Biosystems) and loaded into 48.48 Dynamic Article Title: Apparatus for magnetic separation of cells Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic Single-cell Analysis:Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics. Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq. Lysis:Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics. Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq. cDNA Synthesis:Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics. Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq. Quantitation Assay:Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics. Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq. Gene Expression:Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics. Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq. Sequencing:Article Title: Apparatus for magnetic separation of cells Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic Multiplex Assay:Article Title: Apparatus for magnetic separation of cells Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic Purification:Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Isolation:Article Title: REST represses a subset of the pancreatic endocrine differentiation program Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Control:Article Title: REST represses a subset of the pancreatic endocrine differentiation program Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Expressing:Article Title: Single‐cell analyses reveal functional classification of dendritic cells and their potential roles in inflammatory disease Article Snippet: Dendritic cells (DCs)havecrucial roles in immune-relateddiseases.However, it isdifficult toexploreDCs becauseof their rarenessandheterogeneity.Althoughpreviousstudieshadbeenperformedtodetect thephenotypic characteristics ofDCpopulations, the functionaldiversityhasbeen ignored.Usinga combinationof flowcytometry, single-cell quantitative PCR, and bioinformatic analysis, we depicted the DC panorama with not only phenotypic but also functional markers.. Functional classification of DCs inmouse lymphoid tissue (spleen) and nonlymphoid tissue (liver) was performed.. The results revealed that expression of macrophage scavenger receptor 1 (MSR1) and C–C motif chemokine receptors (CCR)1, CCR2, and CCR4 were elevated in liver DCs, suggesting increased lipid uptake and migration abilities. |