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dynamic array integrated fluidic circuit ifc chips  (fluidigm)


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    fluidigm dynamic array integrated fluidic circuit ifc chips
    Dynamic Array Integrated Fluidic Circuit Ifc Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/48%2E48+dynamic+array+chips/48%2E48+Dynamic+Array+IFC+for+Gene+Expression/pm41866099-141-7-22
    Average 94 stars, based on 313 article reviews
    dynamic array integrated fluidic circuit ifc chips - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: P2x7 receptors control demyelination and inflammation in the cuprizone model
    Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Array chips and processed in the BioMark HD Real-Time PCR System (Fluidigm Corporation, San Francisco, CA, USA). .. We used commercial primers from IDT Integrated DNA Technologies or Fluidigm Corporation.

    Article Title: Apparatus for magnetic separation of cells
    Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic array chips, the NanoFlexTM 4-IFC Controller and the BioMark Real-Time PCR System (Fluidigm Corporation, South San Francisco, Calif.) were used for multiplex qRT-PCR. .. Arrays were performed following the standard Fluidigm protocol (Spurgeon et al., “High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array,” PLoS ONE 3(2): e1662. doi:10.1371 2008).

    Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination
    Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Array chips and processed in the BioMark Real-Time PCR System (Fluidigm Corporation, San Francisco, CA, USA). .. We used commercial primers from IDT Integrated DNA Technologies (Leuven, Belgium) or Fluidigm Corporation targeting Cnr1 (Mm.PT.58.30057922), Cnr 2 (Mm.PT.58.41156189), Rbfox3 (Mm.PT.58.32889417), Aqp4 (Mm.PT.58.9080805), Gfap (Mm.PT.58.31297710), Olig2 (Mm.PT.58.42319010), Pdgfrα (Mm.PT.56a.5639577), Mbp (Mbp_31932_e3) , Gpr34 (Gpr34_36763_i0) , P2ry12 (P2ry6_37046_i1) , Tmem119 (Mm.PT.58.6766267).

    Article Title: REST represses a subset of the pancreatic endocrine differentiation program
    Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Array chips (Fluidigm). .. Gene expression data were prepared for analysis using Fluidigm real-time PCR analysis software (v. 4.1.3) with linear derivative baseline correction, a quality correction set to 0.65, and a melting curve analysis with specific temperature range of individual gene amplicons as well as settings of peak sensitivity to 7 and peak ratio threshold to 0.7.

    Reverse Transcription:

    Article Title: P2x7 receptors control demyelination and inflammation in the cuprizone model
    Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Array chips and processed in the BioMark HD Real-Time PCR System (Fluidigm Corporation, San Francisco, CA, USA). .. We used commercial primers from IDT Integrated DNA Technologies or Fluidigm Corporation.

    Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination
    Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Array chips and processed in the BioMark Real-Time PCR System (Fluidigm Corporation, San Francisco, CA, USA). .. We used commercial primers from IDT Integrated DNA Technologies (Leuven, Belgium) or Fluidigm Corporation targeting Cnr1 (Mm.PT.58.30057922), Cnr 2 (Mm.PT.58.41156189), Rbfox3 (Mm.PT.58.32889417), Aqp4 (Mm.PT.58.9080805), Gfap (Mm.PT.58.31297710), Olig2 (Mm.PT.58.42319010), Pdgfrα (Mm.PT.56a.5639577), Mbp (Mbp_31932_e3) , Gpr34 (Gpr34_36763_i0) , P2ry12 (P2ry6_37046_i1) , Tmem119 (Mm.PT.58.6766267).

    Single Cell:

    Article Title: Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis
    Article Snippet: Reverse transcription and low cycle pre-amplification was performed following addition of Superscript III reverse transcriptase enzyme (Invitrogen, Waltham, MA, USA), Cells Direct reaction mix (Invitrogen), and target gene-specific TaqMan assay (primer/probe) sets (Applied Biosystems) ( ). .. Resultant single cell cDNA was mixed with sample loading agent (Fluidigm, South San Francisco, CA, USA) and Universal PCR Master Mix (Applied Biosystems) and loaded into 48.48 Dynamic Array chips (Fluidigm) along with TaqMan assays and assay loading agent according to the manufacturer’s instructions. .. Products were analyzed on the BioMark reader system (Fluidigm) using a hot start protocol to minimize primer-dimer formation, with 40 quantitative PCR cycles performed.

    Polymerase Chain Reaction:

    Article Title: Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis
    Article Snippet: Reverse transcription and low cycle pre-amplification was performed following addition of Superscript III reverse transcriptase enzyme (Invitrogen, Waltham, MA, USA), Cells Direct reaction mix (Invitrogen), and target gene-specific TaqMan assay (primer/probe) sets (Applied Biosystems) ( ). .. Resultant single cell cDNA was mixed with sample loading agent (Fluidigm, South San Francisco, CA, USA) and Universal PCR Master Mix (Applied Biosystems) and loaded into 48.48 Dynamic Array chips (Fluidigm) along with TaqMan assays and assay loading agent according to the manufacturer’s instructions. .. Products were analyzed on the BioMark reader system (Fluidigm) using a hot start protocol to minimize primer-dimer formation, with 40 quantitative PCR cycles performed.

    Article Title: Apparatus for magnetic separation of cells
    Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic array chips, the NanoFlexTM 4-IFC Controller and the BioMark Real-Time PCR System (Fluidigm Corporation, South San Francisco, Calif.) were used for multiplex qRT-PCR. .. Arrays were performed following the standard Fluidigm protocol (Spurgeon et al., “High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array,” PLoS ONE 3(2): e1662. doi:10.1371 2008).

    Single-cell Analysis:

    Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics.
    Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq.

    Lysis:

    Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics.
    Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq.

    cDNA Synthesis:

    Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics.
    Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq.

    Quantitation Assay:

    Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics.
    Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq.

    Gene Expression:

    Article Title: Recent advances in high-throughput single-cell transcriptomics and spatial transcriptomics.
    Article Snippet: Single-cell RNA sequencing (scRNA-seq) has been developed for characterizing the transcriptome of cells that are rare but of biological significance.. With cell barcoding and microchip technologies, a suite of highthroughput scRNA-seq protocols enable transcriptome profiling in thousands of individual cells at singlecell resolution for classifying cell types, discovering novel cell populations, investigating cellular heterogeneity and elucidating lineage trajectories.. Microchip technologies including microfluidicsand microwell-based platforms play a major role in high-throughput scRNA-seq.

    Sequencing:

    Article Title: Apparatus for magnetic separation of cells
    Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic array chips, the NanoFlexTM 4-IFC Controller and the BioMark Real-Time PCR System (Fluidigm Corporation, South San Francisco, Calif.) were used for multiplex qRT-PCR. .. Arrays were performed following the standard Fluidigm protocol (Spurgeon et al., “High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array,” PLoS ONE 3(2): e1662. doi:10.1371 2008).

    Multiplex Assay:

    Article Title: Apparatus for magnetic separation of cells
    Article Snippet: By eliminating costly, time-consuming RNA purification procedures, it is especially valuable for high-throughput applications and is ideal for gene expression experiments. .. TaqMan Universal Master Mix (Applied Biosystems, Foster City, Calif., [TaqMan Universal Master Mix Reagents provide a PCR mix that may be used with any appropriately designed primer and probe to detect any DNA or cDNA sequence].) and 48.48 dynamic array chips, the NanoFlexTM 4-IFC Controller and the BioMark Real-Time PCR System (Fluidigm Corporation, South San Francisco, Calif.) were used for multiplex qRT-PCR. .. Arrays were performed following the standard Fluidigm protocol (Spurgeon et al., “High Throughput Gene Expression Measurement with Real Time PCR in a Microfluidic Dynamic Array,” PLoS ONE 3(2): e1662. doi:10.1371 2008).

    Purification:

    Article Title: Deregulation of the endocannabinoid system and therapeutic potential of ABHD6 blockade in the cuprizone model of demyelination
    Article Snippet: Following quality control of RNA samples (2100 Bioanalyzer, Agilent Technologies, Santa Clara, CA, USA), cDNA synthesis, pre-amplification and amplification steps were performed at the General Genomics Service of the UPV/EHU. .. For nanofluidic qPCR, the pre-amplified cDNA samples from purified microglia were measured with no reverse transcriptase and no template controls on 48.48 Dynamic Array chips and processed in the BioMark Real-Time PCR System (Fluidigm Corporation, San Francisco, CA, USA). .. We used commercial primers from IDT Integrated DNA Technologies (Leuven, Belgium) or Fluidigm Corporation targeting Cnr1 (Mm.PT.58.30057922), Cnr 2 (Mm.PT.58.41156189), Rbfox3 (Mm.PT.58.32889417), Aqp4 (Mm.PT.58.9080805), Gfap (Mm.PT.58.31297710), Olig2 (Mm.PT.58.42319010), Pdgfrα (Mm.PT.56a.5639577), Mbp (Mbp_31932_e3) , Gpr34 (Gpr34_36763_i0) , P2ry12 (P2ry6_37046_i1) , Tmem119 (Mm.PT.58.6766267).

    Isolation:

    Article Title: REST represses a subset of the pancreatic endocrine differentiation program
    Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Array chips (Fluidigm). .. Gene expression data were prepared for analysis using Fluidigm real-time PCR analysis software (v. 4.1.3) with linear derivative baseline correction, a quality correction set to 0.65, and a melting curve analysis with specific temperature range of individual gene amplicons as well as settings of peak sensitivity to 7 and peak ratio threshold to 0.7.

    Control:

    Article Title: REST represses a subset of the pancreatic endocrine differentiation program
    Article Snippet: .. Ninety five single cells were isolated from E12.5 control (36 cells from 2 pancreata) and REST Fl/Fl-Cre (59 cells from 2 pancreata) embryos, and gene-specific cDNA samples were prepared and pre-amplified using 48 gene primers ( Supplemental Table 2 ). qPCR was performed using 48.48 Dynamic Array chips (Fluidigm). .. Gene expression data were prepared for analysis using Fluidigm real-time PCR analysis software (v. 4.1.3) with linear derivative baseline correction, a quality correction set to 0.65, and a melting curve analysis with specific temperature range of individual gene amplicons as well as settings of peak sensitivity to 7 and peak ratio threshold to 0.7.

    Expressing:

    Article Title: Single‐cell analyses reveal functional classification of dendritic cells and their potential roles in inflammatory disease
    Article Snippet: Dendritic cells (DCs)havecrucial roles in immune-relateddiseases.However, it isdifficult toexploreDCs becauseof their rarenessandheterogeneity.Althoughpreviousstudieshadbeenperformedtodetect thephenotypic characteristics ofDCpopulations, the functionaldiversityhasbeen ignored.Usinga combinationof flowcytometry, single-cell quantitative PCR, and bioinformatic analysis, we depicted the DC panorama with not only phenotypic but also functional markers.. Functional classification of DCs inmouse lymphoid tissue (spleen) and nonlymphoid tissue (liver) was performed.. The results revealed that expression of macrophage scavenger receptor 1 (MSR1) and C–C motif chemokine receptors (CCR)1, CCR2, and CCR4 were elevated in liver DCs, suggesting increased lipid uptake and migration abilities.



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